Posters
« Back
Utility of 405nm-Excitable Dyes in High Content Screening Using an Acumen Explorer Microplate Cytometer
EP20311
Poster Title: Utility of 405nm-Excitable Dyes in High Content Screening Using an Acumen Explorer Microplate Cytometer
Submitted on 19 Dec 2013
Author(s): Sarah Payne, Tristan Cope, Christopher Lupton, Jeffrey T. Hung and Paul Wylie
Affiliations: TTP Labtech, Invitrogen Corporation
Poster Views: 810
View poster »


Poster Information
Abstract: The Acumen Explorer is a fluorescent microplate cytometer equipped with either a 405nm or 488 nm laser. The instrument offers rapid read and analysis times of individual cells within 96-3456 well plates (typically 5-10 minutes), making it compatible with the sustained use of high content methods in primary screens. In addition, small file sizes (>50kB in screening mode) are produced, therefore removing the requirement for expensive data storage solutions in compound screening programs.

In this study, we have used an Acumen Explorer equipped with a violet 405nm laser in conjunction with a selection of shorter-wavelength amine-reactive fluorophores (Invitrogen, Molecular Probes) to demonstrate a greater utility for blue fluorescent probes within high content assays.
Summary: In this study, we have used an Acumen Explorer equipped with a violet 405nm laser in conjunction with a selection of shorter-wavelength amine-reactive fluorophores (Invitrogen, Molecular Probes) to demonstrate a greater utility for blue fluorescent probes within high content assays.Report abuse »
Creative Commons

Related Posters


Chipcytometry: Long-term Sample Storage and Re-Interrogation in Clinical Trial Support
Jon Waterman-Smith, Anja Mirenska

FINGERPRINT 1H- NMR BASED ON THE CHEMICAL PROFILING OF COLOMBIAN RED PROPOLIS
Guillermo Salamanca Grosso; Monica Patricia Osorio Tangarife; David Garcia Mendez; Julien Wist

Genetic Engineering in Male Sterility for Hybrid Variety Development
Abir Hasan Joy

VITVO: Mimicking In Vivo Complexity By The Innovative 3D Model
Olivia Candini1, Giulia Grisendi1, Elisabetta Manuela Foppiani1, Matteo Brogli1, Beatrice Aramini2, Valentina Masciale3, Carlotta Spano1, Tiziana Petrachi4, Elena Veronesi4, Pierfranco Conte5,6, Giorgio Mari1 & Massimo Dominici1,3

A Novel Enzymatic Assay for Determination of Phosphatidylinositol in Biological Samples
Paul Templeton, Kyle C. Schmitt, Grigoriy Tchaga,and Gordon Yan