Posters
« Back
Validation of Factor IIa Assay for Dalteparin Sodium
EP28863
Poster Title: Validation of Factor IIa Assay for Dalteparin Sodium
Submitted on 23 Jul 2018
Author(s): Dr Amitabha De, Kiran Shah, Prajakta Ambre, Jyoti Gupta
Affiliations: KRISHGEN BIOSYSTEMS
Poster Views: 448
View poster »


Poster Information
Abstract: Dalteparin anti-FIIa is a chromogenic assay intended for the quantitative determination of Dalteparin in purified solutions by measurement of factor IIa inhibition activity. The kit can be used as per microtiter plate protocol.

The inhibitory effect of anti-thrombin III (AT-III) on thrombin, factor IIa and other coagulation serine proteases in plasma is increased several thousand-fold by Dalteparin. This inhibition accounts for the anticoagulant effect of Dalteparin.

The quantitative determination of Dalteparin levels by the measurement of their anti-IIa activity is a necessary tool for monitoring treatment efficacy.

The kit and its components were used to run samples of biological solutions and plasma to validate and estimate dalteparin sodium.

It was concluded that the kits were accurate and sensitive for the estimation of dalteparin sodium and dalteparin injection as per USP guidelines.
Summary: Validation of a Chromogenic Assay using Factor IIa for estimation of Dalteprain Sodium and Dalteparin Injection. References: Bioequivalence study of two subcutaneous formulations of dalteparin: randomized, single-dose, two-sequence, two-period, cross-over study in healthy volunteers. C. Gadiko, S. K. Tippabhotla, S. Thota, M. Nakkawar, R. Cheerla, M.R. Betha, and V. Vobalaboina. Journal Drug Assess v.2(1); 2013 PMC4937650.
Pharmacodynamics of Intravenous and Subcutaneous Tinzaparin and Heparin in Healthy Volunteers. Am J Health Syst Pharm. 2001;58(17)
Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


Oral Soft Tissue Lumps and Bumps
Sarah Ahmed Mohamed, Noura Mohamed Kamal, Heba Ahmed Saleh

MALDI Imaging Mass Spectrometry for the study of cardiovascular pathology
Takashi Nirasawa1, Megumi Terada2,3, Hiroko Namba2, Nobuto Kakuda2, Patrick Bruneval3, Hatsue Ishibashi-Ueda4 and Masaya Ikegawa1

Distinct deposition of amyloid-bspecies in brains with Alzheimer’s disease pathology visualized with MALDI imaging mass spectrometry
Nobuto Kakuda1, Tomohiro Miyasaka1, Takashi Nirasawa2, Shigeo Murayama3, Yasuo Ihara1 and Masaya Ikegawa1

Routine Analysis of Host Cell Proteins in Antibody Preparations using PASEF
Stuart Pengelley1, Guillaume Tremintin², Waltraud Evers1, Detlev Suckau1

Endocrine Disruptors and Impact of Nutritional Intervention on Hypothyroid Women - A Study in Kolkata
Swapan Banerjee