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Your Body on Chips: Saves Animals, Saves Lives
Poster Title: Your Body on Chips: Saves Animals, Saves Lives
Submitted on 28 May 2019
Author(s): Saundarya Kunaratnam, Wei Xin Ang, Kah Boon Cheok, Doreen En Qin Yek, Intan Afiqah Mohd Ariff, Nur Adrina Azman, Nurliyana Aqilah Zaidon, Nurul Asyikin Amran, Suh Kuan Fong
Affiliations: Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
This poster was presented at The CUBE, Faculty of Medicine, University of Malaya
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Poster Information
Abstract: In drug discovery, billion dollars are invested in cells and animal experiments for the pharmacodynamics and pharmacokinetics evaluation of a potential new drug. However, these models hardly mimics human and this could sometimes lead to clinical trials failure. Organ-on-chip (OOC) is a microengineered cellular model. It recreates the microenvironment using seeded living human cells and serves as an alternative to animal models. It recapitulates the microarchitecture of a living human organ. It was pioneered by the Wyss Institute for Biologically Inspired Engineering at Harward Univesity in 2010. Different medium containing different cell types are introduced into the opposite microfluidic channels of OOC. A porous polydimethylsiloxane (PDMS) membrane sandwiched by two channels provides the tissue-tissue interface for mimicking biological processes. The transparent PDMS enables direct visualization and quantitative analysis of biological processes. Its flexibility allows recreation of peristaltic motions by applying mechanical forces imitating internal body. On-chip tumor growth has been studied to understand the effects of neovascularization process of in vivo-like cancer growth. Physiological movements like breathing actually affects the drug's effectiveness in treating lung cancer. External mechanical forces applied onto this OOC device rhythmically, stretch and relax the cells/tissues interfaces to mimic physiological breathing. The ability of co-culture in OOC through seeding of normal parenchyma and extracellular matrix at two different channels, revealed the role of extracellular matrix and interaction in promoting cancer metastasis, which further identified how they influence growth of cancers. This is very useful in targeting cancer therapy research. The in vivo-like microenvironment in OOC provides a better platform for evaluating anticancer therapies to its highest efficacy with lowest cytotoxicity. In future, researchers attempt to implement this technology with individual patients’ cells for advancing personalized medicine. In conclusion, OOC is a splendid invention to replace animal testing in clinical research for the understanding of human pathophysiology.Summary: A multi-channel 3-D microfluidic cell culture chip, which constitutes of biomedical microelectromechanical systems to represent key functional units of living human organs.References: Caballero, D., Kaushik, S., Correlo, V. M., Oliveira, J. M., Reis, R. L., & Kundu, S. C. (2017). Organ-on-chip models of cancer metastasis for future personalized medicine: From chip to the patient. Biomaterials, 149, 98-115.
Huh, D., Hamilton, G. A., & Ingber, D. E. (2011). From 3D cell culture to organs-on-chips. Trends in cell biology, 21(12), 745-754.
Perestrelo, A., Águas, A., Rainer, A., & Forte, G. (2015). Microfluidic organ/body-on-a-chip devices at the convergence of biology and microengineering. Sensors, 15(12), 31142-31170.
Ronaldson-Bouchard, K., & Vunjak-Novakovic, G. (2018). Organs-on-a-chip: a fast track for engineered human tissues in drug development. Cell Stem Cell, 22(3), 310-324.
Sontheimer-Phelps, A., Hassell, B. A., & Ingber, D. E. (2019). Modelling cancer in microfluidic human organs-on-chips. Nature Reviews Cancer, 1.
Sung, K. E., & Beebe, D. J. (2014). Microfluidic 3D models of cancer. Advanced Drug Delivery Reviews, 79, 68-78.
Tsai, H.
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